The Relation between CagPAI Integrity and the Polymorphisms in the CagA Gene with the Severity of Gastritis in Patients Infected With CagA-Positive H. pylori
Keywords:
Helicobacter Pylori, gastritis, virulence factors, polymorphism, geneticAbstract
Objectives: To evaluate gastric histopathology in Colombian patients infected with CagA-positive Helicobacter pylori and its association with the integrity of Cag (CagPAI) pathogenicity island and the number of EPIYA-C motifs present in the CagA protein.
Methods: Thirty-one (31) individuals diagnosed with gastritis were included in the study. Using gastric biopsies, CagA-positive H.pylori was isolated and EPIYA motif makeup was characterized by sequencing. Histopathology was evaluated with updated Sydney system. The CagA, CagT, CagE and Cag10 genes were genotyped using PCR and agarose electrophoresis.
Results: A total of 24 isolates (77% of cases) carried a complete CagPAI. Among the negative isolates for one or more genes with CagPAI, 7 (22%) were considered to be carriers of a defective CagPAI. No significant differences were observed in H. pylori density averages, degree of chronic inflammation, presence of glandular atrophy or intestinal metaplasia between isolates with complete CagPAI and isolates with defective CagPAI. No significant differences were observed in the histopathological parameters between isolates with one EPIYA-C motif and those with more than one EPIYA-C motif, neither in antrum nor in body, except for infiltration by neutrophils which was significantly greater in bodies with isolates with more than one EPIYA-C motif (p=0.018).
Conclusions: No association was found between the diversity of virulence CagPAI and CagA factors in Colombian isolates and the histopathological findings in gastritis, or in other host or environmental factors that could affect the histopathological characteristics of gastritis.
Author Biographies
María M. Bravo, Instituto Nacional de Cancerología
Grupo de Investigación en Cáncer y Agentes Infecciosos, Instituto Nacional de Cancerología, Bogotá, D. C., Colombia
Teresa Martínez, Instituto Nacional de Cancerología
Grupo Investigación Epidemiológica, Instituto Nacional de Cancerología, Bogotá, D. C., Colombia
Juan C. Bravo, Fundación Valle del Lili
Departamento de Patología, Fundación Valle del Lili, Santiago de Cali, Valle, Colombia
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