Pre-cancerous gastric lesions in Colombian patients: association with IL-1B-511, IL-1RN, IL-10-919, IL-10-1082, TNF-a-308 genes polymorphisms, and anti-Helicobacter pylori cagA IgG antibodies
Keywords:
Interleukin-1beta, Interleukin 1 receptor antagonist, Interleukin-10, Tumor necrosis factor-alpha, Helicobacter pylori, Pre-cancerous gastric lesionAbstract
Objective: To evaluate the relationship of some of the most studied cytokines (IL-1B-511, IL-1RN intron-2-VNTR, TNF-α-308, IL-10-819, and IL-10-1082) with gastric cancer, as well as the presence of anti-Helicobacter pylori cagA IgG antibodies with pre-cancerous lesions in Colombian patients.
Materials and methods: A study was conducted on 185 patients with pre-cancerous lesions (atrophic gastritis, intestinal metaplasia and dysplasia), and 154 controls (non-atrophic gastritis), seen in hospitals in a high risk area, and another in a low risk area, for gastric cancer. Gastric biopsy specimens and blood samples were obtained. The genotyping of the polymorphisms was performed by allelic discrimination using real-time PCR, conventional PCR, and agarose electrophoresis (VNTR of IL-1RN intron 2). The serology of Helicobacter pylori and Helicobacter pylori cagA was determined by ELISA. A multinomial logistic regression was used in the statistical analysis.
Results: The IL-1B-511TT genotype was associated with intestinal metaplasia (OR=4.05; 95% CI; 1.35-12.10). No other relationships were observed between the different polymorphisms and pre-cancerous lesions. Infection due to a positive Helicobacter pylori cagA was associated with atrophic gastritis, intestinal metaplasia and dysplasia (OR=2.66; 13.70; 40.29, respectively).
Conclusion: The results suggest that, among the pro-inflammatory genotypes, the IL-1B-511TT would be associated with intestinal metaplasia, and that a positive Helicobacter pylori cagA serology could be a useful biomarker for the intervention and prevention of pre-cancerous lesions. Further studies are required in the Colombian population in order to evaluate the relationship found between IL1B-511 and intestinal metaplasia.
Author Biographies
Teresa Martínez, Instituto Nacional de Cancerología ESE
Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Gustavo A. Hernández, Instituto Nacional de Cancerología ESE
Grupo de Investigación Epidemiológica, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
María Mercedes Bravo, Instituto Nacional de Cancerología ESE
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Esperanza Trujillo, Instituto Nacional de Cancerología ESE
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología ESE., Bogotá, D.C., Colombia
Jesús Pérez García, Universidad Libre
Facultad de Medicina, Universidad Libre, Laboratorio de Patología Clínica General del Norte, Barranquilla, Colombia
Juan C. Robayo, Fundación Santa Fe de Bogotá
Departamento de Gastroenterología, Fundación Santa Fe de Bogotá, Bogotá, D.C., Colombia
Margarita Camorlinga, Instituto Mexicano de Seguros Sociales
Laboratorio de Bacteriología, Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría CMN siglo XXI, Instituto Mexicano de Seguros Sociales, Ciudad de México, México
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