Daño del ADN como marcador potencial en el seguimiento clínico de pacientes femeninas con cáncer tratado

Autores/as

  • Judith Beatriz Pupo Balboa Laboratorio de Estrés Oxidativo. Centro Nacional de Genética Médica. Dirección: Ave. 31 Esq.146 No 3102, Reparto Cubanacán, CP 11400, La Habana. Cuba. https://orcid.org/0000-0002-6682-0277
  • Martha Robaina Castellanos Servicio de Ontogenética. Instituto Nacional de Oncología y Radiobiología. Dirección: Calle 29 esquina F, Vedado, Plaza de la Revolución, CP 10400, La Habana, Cuba. https://orcid.org/0000-0002-5975-6190
  • Reinaldo Gutiérrez-Guriérrez Laboratorio de Estrés Oxidativo. Centro Nacional de Genética Médica. Dirección: Ave. 31 Esq.146 No 3102, Reparto Cubanacán, CP 11400, La Habana. Cuba. https://orcid.org/0000-0001-6730-0647
  • Anamarys Pandolfi-Blanco Laboratorio de Estrés Oxidativo. Centro Nacional de Genética Médica. Dirección: Ave. 31 Esq.146 No 3102, Reparto Cubanacán, CP 11400, La Habana. Cuba. https://orcid.org/0000-0002-2252-2067
  • Lucia Fariñas Rodríguez Centro Nacional de Genética Médica https://orcid.org/0000-0003-0576-7190

DOI:

https://doi.org/10.35509/01239015.122

Palabras clave:

Cáncer, Ensayo cometa alcalino, Ensayo cometa neutral, Daño del ADN
Resumen Autores/as Referencias bibliográficas Cómo citar

Resumen

Objetivo: Identificar diferencias individuales en el daño basal (DB) del ADN de leucocitos periféricos de mujeres con cáncer en remisión. Métodos: Estudio analítico de corte transversal en el que participaron 24 mujeres con cáncer en remisión de diferentes localizaciones y 24 mujeres supuestamente sanas. Se utilizó el ensayo cometa alcalino y la variante neutral para determinar roturas de simple hebra (DB-A), y roturas de doble hebra del ADN (DB-N), respectivamente.
Resultados: Aunque no hubo diferencias entre los valores medios del daño del ADN de pacientes y controles (DB-N: p=0,43 y DB-A: p=0,13), el 41,6% de las pacientes presentó aumento de un tipo u otro de roturas del ADN, respecto a los correspondientes puntos de corte de las mujeres controles. El DB-N estuvo correlacionado con el incremento de la edad (r2 = 0,1833; r = 0,4281; p = 0,036) en las pacientes. El DB-A estuvo elevado en aquellas que habían recibido politerapia anticáncer (p = 0,024) y en las que estaban realizando tratamiento con tamoxifeno (p=0,033); mientras estuvo disminuido en las que consumieron antioxidantes (p=0,006) y en las que combinaron tamoxifeno y antioxidantes (p=0,020). Conclusiones: Se identificaron diferencias individuales en ambos tipos de roturas de hebra del ADN que resultan de interés médico en las pacientes estudiadas. El daño basal del ADN determinado por ensayo cometa es una
herramienta potencial en el seguimiento clínico de pacientes con cáncer en remisión.

Biografía del autor/a

Judith Beatriz Pupo Balboa, Laboratorio de Estrés Oxidativo. Centro Nacional de Genética Médica. Dirección: Ave. 31 Esq.146 No 3102, Reparto Cubanacán, CP 11400, La Habana. Cuba.

Ph.D..National Center of Medical Genetic, Oxidaive stress

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Cómo citar

[1]
Pupo Balboa, J. et al. 2020. Daño del ADN como marcador potencial en el seguimiento clínico de pacientes femeninas con cáncer tratado. Revista Colombiana de Cancerología. 25, 1 (mar. 2020). DOI:https://doi.org/10.35509/01239015.122.

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Publicado

12-03-2020

Número

Vol. 25 Núm. 1 (2021)

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Artículos de investigación/originales

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