Polimorfismos genéticos de interleucinas IL-1B-511, IL-1RN, IL-10, factor de necrosis tumoral α-308 e infección por Helicobacter pylori CagA positivo en cáncer gástrico y úlcera duodenal en diferentes poblaciones en Colombia
Palabras clave:
Interleucinas 1B, 10, factor de necrosis tumoral alpha, Helicobacter pylori, proteína cagA, neoplasias de estomago, ulcera duodenalResumen
Objetivo: Determinar la asociación entre los polimorfismos IL-1B-511, IL-1RN, TNF-α-308, IL-10-819 e IL-101082 y la infección por Helicobacter pylori CagA positivo en un grupo de pacientes con cáncer gástrico y úlcera duodenal en diferentes poblaciones en Colombia.
Métodos: Estudio de casos y controles con 341 pacientes: con gastritis no atrófica, 194; con cáncer gástrico, 58; úlcera duodenal con lesiones preneoplásicas, 54; y con úlcera duodenal, 35. La genotipificación de los polimorfismos se hizo por discriminación alélica usando PCR en tiempo real, y la del IL-1RN, por PCR convencional y electroforesis en agarosa. La infección por Helicobacter pylori CagA se determinó mediante ELISA. Se utilizó la regresión logística en el análisis estadístico.
Resultados: Ser portador del genotipo IL-1B-511TT (OR=4,69; IC 95% 1,22-18,09) y tener una infección por Helicobacter pylori CagA positivo (OR=4,43; IC 95% 1,72-11,4) se asociaron a cáncer gástrico. Tener una infección por Helicobacter pylori CagA positivo (OR=4,39; IC95% 1,82-10,59) se asoció a la presencia de úlcera duodenal con lesiones preneoplásicas, y ser portador del genotipo IL-1B-511CT se asoció a úlcera duodenal (OR=0,30; IC 95% 0,10-0,91).
Conclusión: Los resultados sugieren que la respuesta pro-inflamatoria y la genética virulenta de la bacteria son factores relacionados con los diferentes desenlaces ocasionados por la infección por Helicobacter pylori en la población estudiada; así, el polimorfismo IL-1B-511 es un factor relacionado con cáncer gástrico y úlcera duodenal, y la infección por Helicobacter pylori CagA positivo es un factor asociado a cáncer gástrico y úlcera duodenal con lesiones preneoplásicas.
Biografía del autor/a
Teresa Martínez, Instituto Nacional de Cancerología
Grupo de Investigación Epidemiológica del Cáncer, Instituto Nacional de Cancerología, Bogotá D.C., Colombia
Gustavo Hernández, Instituto Nacional de Cancerología
Grupo de Investigación Epidemiológica del Cáncer, Instituto Nacional de Cancerología, Bogotá D.C., Colombia
María M. Bravo, Instituto Nacional de Cancerología
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D.C., Colombia
Esperanza Trujillo, Instituto Nacional de Cancerología
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D.C., Colombia
Andrés Quiroga, Instituto Nacional de Cancerología
Grupo de Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D.C., Colombia
Juan C. Robayo, Fundación Santa Fe
Departamento de Gastroenterología, Fundación Santa Fe, Bogotá D. C., Colombia
Jesús Pérez, Clínica General del Norte
Grupo de investigación, Clínica General del Norte, Barranquilla, Colombia
Juan C. Bravo, Fundación Valle del Lili
Departamento de Patología, Fundación Valle del Lili, Cali, Valle, Colombia
Margarita Camorlinga, Instituto Mexicano de Seguros Sociales
Hospital de Pediatría CMN Siglo XXI, Instituto Mexicano de Seguros Sociales, Ciudad de México, México
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