Análisis de la población de células asesinas naturales (NK) en sangre periférica y en linfocitos infiltrantes de tumor en pacientes con cáncer de cuello uterino
Palabras clave:
linfocitos infiltrantes de tumor, células asesinas naturales, neoplasias de cuello uterinoResumen
Objetivo: Entender la importancia biológica y clínica de las células intratumorales natural killer (NK) CD16+CD56+CD3- y de las células natural killer T (NKT) CD16+CD56+CD3- en la inmunovigilancia del cáncer de cuello uterino (CCU).
Métodos: Para comprender el papel de las NK (CD16+CD56+CD3-) y de las células natural killer T (NKT) (CD16+CD56+CD3-) en la inmunovigilancia del CCU, se analizaron 39 muestras de sangre periférica (SP) y 30 biopsias de pacientes con CCU, así como de 40 muestras de SP y 5 biopsias de cuello uterino de mujeres con citología normal. Las frecuencias de NK y NKT y la expresión de HLA-I se analizaron por citometría de flujo.
Resultados: Se observó una mayor frecuencia de NK en SP en el grupo de pacientes comparado con el grupo control (p = 0,002). Sin embargo, este aumento no se reflejó en TIL (p = 0,095). Una reducción significativa de HLA-I se observó en el grupo de pacientes (p = 0,019). Esta disminución se asoció una disminución en el número de NK, pero no fue significativa (p = 0,374). Un bajo número de NK se asoció con una menor supervivencia, pero no fue significativo (p = 0,275).
Conclusiones: Nuestros resultados muestran que aunque en SP se observa un incremento de NK, este no se refleja en los TIL. Es posible que este tráfico ineficiente de células NK hacia el tumor esté alterado por la expresión de citoquinas inmunosupresoras, en particular IL-10.
Biografía del autor/a
María A. Céspedes, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Josefa A. Rodríguez, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Mónica Medina, Instituto Nacional de Cancerología
Clínica de Ginecología, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
María Bravo, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Alba L. Cómbita, Instituto Nacional de Cancerología
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá D. C., Colombia
Departamento de Microbiología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá D. C., Colombia
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