Distribución de variantes del virus del papiloma humano 16 (VPH 16) en mujeres con y sin neoplasia intraepitelial cervical grado 3 y cáncer cervical
Palabras clave:
Neoplasias del cuello uterino, Papilomavirus Humano 16, sondas de ADN de HPV, adenocarcinomaResumen
Objetivos: Describir la distribución de variantes del virus del papiloma humano 16 en mujeres con y sin neoplasia intraepitelial cervical grado 3 y cáncer cervical.
Métodos: Se determinaron las variantes moleculares en casos de carcinoma escamocelular, adenocarcinoma cervical y en mujeres sin anormalidades citológicas de alto grado y positivas para el virus del papiloma humano 16. Para la detección de las variantes moleculares se amplificó el marco abierto de lectura del gen E6 del virus del papiloma humano 16 y se utilizó una técnica de hibridación reversa para la detección de los principales cambios de nucleótidos que identifican las ramas filogenéticas y las clases de variantes.
Resultados: Hubo diferencias estadísticamente significativas en la distribución de variantes de virus del papiloma humano 16. Los controles no presentaron infecciones con variantes no europeas, mientras que ellas estuvieron presentes en el 30% de los casos de carcinoma escamocelular o neoplasia intraepitelial cervical grado tres. En adenocarcinoma, el 65% de las infecciones fueron del tipo no europeo.
Conclusiones: La prevalencia de variantes no europeas de virus de papiloma humano 16 fue de 31,2% en neoplasia intraepitelial cervical grado 3 y cáncer escamocelular, y de 64,1% en adenocarcinoma de cérvix, mientras que estas no se observaron en mujeres sin cáncer.
Biografía del autor/a
Esteban Lopera, Universidad de Antioquia
Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Patricia Acosta, Universidad del Cauca
Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Yaliana Tafurt, Universidad del Cauca
Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Mary Uribe, Universidad de Antioquia
Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Carlos Córdoba, Hospital Universitario San Vicente de Paúl
Departamento de Ginecología y Obstetricia, Hospital Universitario San Vicente de Paúl, Medellín, Colombia
Piedad Acosta, Universidad del Cauca
Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Departamento de Ginecología y Obstetricia, Facultad Ciencias de la Salud, Universidad del Cauca, Popayán, Colombia
Katherine Quintero, Universidad de Antioquia
Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Yexania Arboleda, Universidad del Cauca
Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Hernán Sierra, Universidad del Cauca
Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Gloria Sánchez, Universidad de Antioquia
Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Astrid Bedoya, Universidad de Antioquia
Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia
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