Distribución de variantes del virus del papiloma humano 16 (VPH 16) en mujeres con y sin neoplasia intraepitelial cervical grado 3 y cáncer cervical

Autores/as

  • Esteban Lopera Universidad de Antioquia
  • Patricia Acosta Universidad del Cauca
  • Yaliana Tafurt Universidad del Cauca
  • Mary Uribe Universidad de Antioquia
  • Carlos Córdoba Hospital Universitario San Vicente de Paúl
  • Piedad Acosta Universidad del Cauca
  • Katherine Quintero Universidad de Antioquia
  • Yexania Arboleda Universidad del Cauca
  • Hernán Sierra Universidad del Cauca
  • Gloria Sánchez Universidad de Antioquia
  • Astrid Bedoya Universidad de Antioquia

Palabras clave:

Neoplasias del cuello uterino, Papilomavirus Humano 16, sondas de ADN de HPV, adenocarcinoma

Resumen

Objetivos: Describir la distribución de variantes del virus del papiloma humano 16 en mujeres con y sin neoplasia intraepitelial cervical grado 3 y cáncer cervical.
Métodos: Se determinaron las variantes moleculares en casos de carcinoma escamocelular, adenocarcinoma cervical y en mujeres sin anormalidades citológicas de alto grado y positivas para el virus del papiloma humano 16. Para la detección de las variantes moleculares se amplificó el marco abierto de lectura del gen E6 del virus del papiloma humano 16 y se utilizó una técnica de hibridación reversa para la detección de los principales cambios de nucleótidos que identifican las ramas filogenéticas y las clases de variantes.
Resultados: Hubo diferencias estadísticamente significativas en la distribución de variantes de virus del papiloma humano 16. Los controles no presentaron infecciones con variantes no europeas, mientras que ellas estuvieron presentes en el 30% de los casos de carcinoma escamocelular o neoplasia intraepitelial cervical grado tres. En adenocarcinoma, el 65% de las infecciones fueron del tipo no europeo.
Conclusiones: La prevalencia de variantes no europeas de virus de papiloma humano 16 fue de 31,2% en neoplasia intraepitelial cervical grado 3 y cáncer escamocelular, y de 64,1% en adenocarcinoma de cérvix, mientras que estas no se observaron en mujeres sin cáncer.

Biografía del autor/a

Esteban Lopera, Universidad de Antioquia

Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia

Patricia Acosta, Universidad del Cauca

Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia

Yaliana Tafurt, Universidad del Cauca

Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia

Mary Uribe, Universidad de Antioquia

Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia

Carlos Córdoba, Hospital Universitario San Vicente de Paúl

Departamento de Ginecología y Obstetricia, Hospital Universitario San Vicente de Paúl, Medellín, Colombia

Piedad Acosta, Universidad del Cauca

Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia
Departamento de Ginecología y Obstetricia, Facultad Ciencias de la Salud, Universidad del Cauca, Popayán, Colombia

Katherine Quintero, Universidad de Antioquia

Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia

Yexania Arboleda, Universidad del Cauca

Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia

Hernán Sierra, Universidad del Cauca

Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia

Gloria Sánchez, Universidad de Antioquia

Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia

Astrid Bedoya, Universidad de Antioquia

Grupo Infección y Cáncer, Universidad de Antioquia, Medellín, Colombia
Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia

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Cómo citar

[1]
Lopera, E. et al. 2012. Distribución de variantes del virus del papiloma humano 16 (VPH 16) en mujeres con y sin neoplasia intraepitelial cervical grado 3 y cáncer cervical. Revista Colombiana de Cancerología. 16, 4 (dic. 2012), 205–216.

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Publicado

01-12-2012

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Artículos de investigación/originales