Daño del ADN como marcador potencial en el seguimiento clínico de pacientes femeninas con cáncer tratado

Daño del ADN en cáncer tratado

  • Judith Beatriz Pupo Balboa National Center of Medical Genetic https://orcid.org/0000-0002-6682-0277
  • Martha Robaina Castellanos Instituto Nacional de Oncología y Radiobiología
  • Reinaldo Gutiérrez-Guriérrez Centro Nacional de Genética Médica
  • Anamarys Pandolfi-Blanco Centro Nacional de Genética Médica
  • Lucia Fariñas Rodríguez Centro Nacional de Genética Médica
Palabras clave: Cáncer; Ensayo cometa alcalino; Ensayo cometa neutral; Daño del ADN

Resumen

Objetivo: Identificar diferencias individuales en el daño basal (DB) del ADN de linfocitos periféricos de mujeres con cáncer en remisión. Métodos: Estudio analítico de corte transversal en el que participaron 24 mujeres con cáncer en remisión de diferentes localizaciones y 24 mujeres supuestamente sanas. Se utilizó el ensayo cometa alcalino y la variante neutral para determinar roturas de simple hebra (DB-A), y roturas de doble hebra del ADN (DB-N), respectivamente. Resultados: Aunque no hubo diferencias entre los valores medios del daño del ADN de pacientes y controles (DB-N: p=0,43 y DB-A: p=0,13), el 41,6% de las pacientes presentó aumento de un tipo u otro de roturas del ADN y el DB-N estuvo asociado al incremento de la edad (r2 = 0,1833; r = 0,4281; p = 0,036) en este grupo. El consumo de antioxidantes pareció contribuir a un DB-A menor (p=0,006) en un grupo de pacientes, semejante al grupo control (p=0,013), respecto a las que no consumieron los suplementos. El tratamiento con tamoxifeno pareció incrementar el DB-A en las pacientes con cáncer de mama en remisión que no consumieron antioxidantes, en relación a las que si consumieron el medicamento (p=0,020) y al grupo control (p=0,033). Conclusiones: Se identificaron diferencias individuales en ambos tipos de roturas de hebra del ADN que resultan de interés médico en las pacientes estudiadas. El daño basal del ADN determinado por ensayo cometa es una herramienta potencial en el seguimiento clínico de pacientes con cáncer en remisión para determinar la presumible susceptibilidad al cáncer secundario.

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Biografía del autor/a

Judith Beatriz Pupo Balboa, National Center of Medical Genetic

Ph.D..National Center of Medical Genetic, Oxidaive stress

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Publicado
2020-12-02
Cómo citar
[1]
Pupo Balboa, J., Castellanos, M.R., Gutiérrez-Guriérrez, R., Pandolfi-Blanco, A. y Fariñas Rodríguez, L. 2020. Daño del ADN como marcador potencial en el seguimiento clínico de pacientes femeninas con cáncer tratado : Daño del ADN en cáncer tratado. Revista Colombiana de Cancerología. 25, 1 (dic. 2020). DOI:https://doi.org/10.35509/01239015.122.
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Artículos de investigación/originales